Andrii Chobotarov, Mykola Volkogon, Lesya Voytenko, Ivan Kurdish
Microbiology of Microbiology
Azotobacter vinelandii IMV B-7076 was shown to be capable of biosynthesis and accumulation of various biologically active substances, including high amounts of 3-indoleacetic acid (IAA) in free and bound forms, in the cultural medium. Cultivation of these bacteria with vermiculite particles had increased accumulation of abscisic acid (ABA) in 2.3 times compared to the control. Addition of silica dioxide nanoparticles to the nutrient medium had resulted in higher cytokinin content. Thus, the detected amount of zeatin was 2.4 times higher; zeatin-riboside – higher in 3.6 times; while the content of zeatin-glucoside had a slight increase by 3%.
High amounts of cytokinins, 3-indoleacetic and abscisic acids were also detected in the cultural medium of Bacillus subtilis IMV B-7023. Bacteria cultivation in the presence of vermiculite particles in nutrient medium had stimulated an increase of isopentyl adenine content in 4.0 times compared to control. Substantial (in 4.5 times) increase of 3-indoleacetic acid synthesis compared to control was detected. Addition of nanoparticles to the nutrient medium of Bacillus subtilis IMV B-7023 had also promoted synthesis of the abscisic acid in 1.7 times compared to control.