Faculty of Biotechnology and Food Sciences in Nitra Journal of Microbiology, Biotechnology and Food Sciences 1338-5178 special issue 1 (Microbiology) vol. 4 2015-02-02 2015-02-02 LIME PRETREATMENT OPTIMIZATION OF WHEAT STRAW TO IMPROVE ETHANOL PRODUCTION BY SACCHAROMYCES CEREVISIAE AND ITS VALIDATION 45 47 EN Miroslav Ondrejovič Miroslav Ondrejovič Veronika Dudášová Veronika Dudášová Daniela Chmelová Daniela Chmelová The aim of this study was to optimize of independent variables as temperature, time and reaction ratio to output parameter of simultaneous enzyme saccharification and fermentation by Saccharomyces cerevisiae of pretreated wheat straw as model substrate via RSM (response surface methodology) approach. As dependent variable, it was chosen ethanol yields characterizing effectivity of process. The optimal conditions were approximately temperature 100 °C, time 1 hour and reaction ratio 26 mL to 1 g of treated wheat straw with ethanol yields 141.9 mg.g-1. After calculating the optimal values, the validation analyze was carried out and it was found out that the predicted and experimentally verified dependent variable was in agreement with the optimal parameters (~ 95 %). Proposed model was tested for three lignocellulosic materials (winter wheat straw, alfalfa hay and maize straw) as wheat straw used as model substrate and it was confirmed the possibility of its use for other agricultural residues with similar content of lignocellulose.
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