Faculty of Biotechnology and Food Sciences in Nitra Journal of Microbiology, Biotechnology and Food Sciences 1338-5178 special issue 1 (Microbiology) vol. 4 2015-02-02 2015-02-02 IMPACT OF PESTICIDES USED IN THE CULTURE OF THE VINE ON THE VIABILITY OF THE YEAST SACCHAROMYCES CEREVISIAE WINE IN CHRONOLOGICAL AGING 48 51 EN Alina Owsiak Alina Owsiak Magdalena Marchel Magdalena Marchel Ewa Zyracka Ewa Zyracka Pesticides, used in culture, may induce oxidative stress by stimulation of free radicals production, what may result in lipid peroxidation, proteome damage, changes in DNA and RNA structures and disturbance of total antioxidative capacity in organisms’ cells. In disturbances caused by increase synthesis ROS (reactive oxygen species) or lack antioxidative defense that is in oxidative stress it is seen one of all causes of aging process. Chronological aging of baker’s and wine yeast Saccharomyces cerevisiae in liquid stationary culture is used as model of research on the aging process. As a result of aging changes take place in yeast cells which have physiological, genetic, metabolic and morphological character, what cause their death in consequence. Some scientists treat chronological yeast aging as analogy of fibroblasts aging of multicellular organisms, skeletal muscles or nerve cells. The aim of the experiment was to obtain the answer on question connected with toxicity effect two widely available pesticides in shape of trade preparation, used among other things in culture of grapevine Miedzian 50 WP (Cu 50WP) and Siarkol Extra 80 WP (S 80WP) on vitality of wine yeast in chronological aging. During research cells of wine yeast Tokay, which are used in production of white wines, and cells of Malaga strain, used in production of white and red wines, were applied. Yeast culture with pesticides supplementation in determined concentrations was conducted through seven days in YPG medium. At that time vitality of yeast cells was determined by the percentage of cells surviving, percentage of dead cells and culture density. Considerable influence on decreasing vitality of yeast cells in the process of aging showed S 80WP, what correlate with the increase of applied concentration in both example of Malaga and Tokay. Obtained results in application of Cu 50WP indicated lower toxicity in culture of both studied strains in comparison to the control. Our studies suggest that studied pesticides may cause the intensity of processes connected with cells aging.
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