Faculty of Biotechnology and Food Sciences in Nitra Journal of Microbiology, Biotechnology and Food Sciences 1338-5178 special issue 2 (Biotechnology) vol. 4 2015-02-02 2015-02-02 ASSESSMENT OF T-2 TOXIN EFFECT AND ITS COMBINATION WITH GROWTH FACTOR AND METABOLIC HORMONES ON 17β-ESTRADIOL SECRETION BY RABBIT OVARIAN FRAGMENTS IN VITRO 51 53 EN Adriana Kolesárová Adriana Kolesárová Nora Maruniaková Nora Maruniaková Attila Kádasi Attila Kádasi Dagmara Packová Dagmara Packová Marek Halenár Marek Halenár Alexander V. Sirotkin Alexander V. Sirotkin Jozef Bulla Jozef Bulla Assessment of T-2 effect and its combination with growth factor IGF-I, metabolic hormones leptin and ghrelin on 17β-estradiol secretion by rabbit ovarian fragments was studied. Rabbit ovarian fragments were incubated without (control group) or with alone T-2 toxin (0.01; 0.1; 1; 10; and 100 ng.mL-1), or its combination with insulin-like growth factor I - IGF-I (100 ng.mL-1), leptin (1000 ng.mL-1) or ghrelin (500 ng.mL-1) for 24 hours. Secretion of 17β-estradiol was determined by ELISA. T-2 toxin was not shown to be potential regulator of 17β-estradiol secretion in rabbit ovarian fragments. On the other hand T-2 toxin at all used doses combined with IGF-I significantly (P<0.05) inhibited 17β-estradiol secretion by the fragments. Similarly, 17β-estradiol secretion was significantly (P<0.05) inhibited by T-2 toxin at the highest used doses (10 and 100 ng.mL-1) combined with leptin (1000 ng.mL-1). On the other hand T-2 toxin combined with ghrelin was not shown to be potential regulator of 17β-estradiol secretion in rabbit ovarian fragments. Results in this study showed that trichothecene as T-2 toxin combined with IGF-I or leptin was able to modulate 17β-estradiol secretion in rabbit ovarian fragments in vitro. We suggest their possible involvement to the process of steroidogenesis.
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