Faculty of Biotechnology and Food Sciences in Nitra Journal of Microbiology, Biotechnology and Food Sciences 1338-5178 special issue 2 (Biotechnology) vol. 4 2015-02-02 2015-02-02 SEQUENCE ANALYSIS OF SUNDEW CHITINASE GENE 4 6 EN Dominika Ďurechová Dominika Ďurechová Ildikó Matušíková Ildikó Matušíková Jana Moravčíková Jana Moravčíková Martin Jopčík Martin Jopčík Jana Libantová Jana Libantová Progress in biotechnology has generated an interest towards the isolation and characterization of genes that should be used in strengthening of plant defence against biotic and abiotic stresses. Here we focused on sequence analysis of the chitinase gene isolated from untraditional genetic source, carnivorous plant sundew (DroserarotundifoliaL.). The genomic sequence of DrChit indicated the coding region contained three exons and two introns.Splicing of introns predicted in silicowas confirmed by experimental approach. Moreover, RACE (rapid amplification cDNA ends) analysis revealed the transcription start sitelocated 41 bp upstream of the ATG start codon. This is one of the first reports onthe isolation and molecular characterization of chitinase gene from the carnivorous sundew plant.
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