Faculty of Biotechnology and Food Sciences in Nitra Journal of Microbiology, Biotechnology and Food Sciences 1338-5178 special issue 2 (Biotechnology) vol. 4 2015-02-02 2015-02-02 THE DIFFERENCES BETWEEN THE OLD AND NEW BARLEY VARIETIES ON THE BASIS OF HORDEIN POLYMORPHISM WITH RESPECT TO QUALITATIVE PARAMETERS 108 110 EN Marián Tomka Marián Tomka Milan Milan Chňapek Chňapek Zdenka Gálová Zdenka Gálová Dana Urminská Dana Urminská The current registered barley varieties are classified according to their biological and economic characters meeting the growing conditions. Varieties are characterized using molecular markers on the level of protein and DNA. Using starch gel electrophoresis we studied the frequency of hordein alleles, which are potential markers of economically important traits of 30 barley varieties. Among the 26 alleles detected in two main loci (Hrd A, Hrd B), we identified 7 potential markers (A3, A12, A23, B8, B17, B19, B47) with the frequency ranged from 1.18% to 12.14%. Number of these alleles differs over the years and together with number of lines per variety has decreasing tendency. On the basis of the detected alleles we are able to predict malting quality parameters and resistance to most common barley pathogens.