Faculty of Biotechnology and Food Sciences in Nitra Journal of Microbiology, Biotechnology and Food Sciences 1338-5178 no. 5 vol. 4 2015-04-01 2015-04-01 USE OF BUTTER MILK AND POULTRY-TRANSFORMING WASTES FOR ENHANCED PRODUCTION OF Bacillus subtilis SPB1 BIOSURFACTANT IN SUBMERGED FERMENTATION 462 466 EN Raida Zouari Raida Zouari Semia Ellouze-Chaabouni Semia Ellouze-Chaabouni Dhouha Ghribi Dhouha Ghribi Biosurfactants are valuable microbial amphiphilic molecules with effective surface-active and biological properties applicable to several industries and processes. Microorganisms synthesize them, especially during growth on water-immiscible substrates, providing an alternative to chemically prepared conventional surfactants. Microbial surfactants are not yet a sustainable alternative to chemically synthesized surfactants seeing their potentially high production charges. This study highlights the use of low-cost agro-industrial raw material for fermentative production of biosurfactants. The Box–Behnken Design and response surface methodology were employed to optimize the concentrations of the ratio butter milk /distilled water, poultry-transforming wastes and inoculum size for lipopeptide biosurfactant production by B.subtilis SPB1 in submerged fermentation.The best production yield was about 12.61 ± 0.7 g/L of crude lipopeptide biosurfactant. It can be obtained when using a ratio butter milk /distilled water of 1.5, poultry-transforming wastes of 23g/L and an inoculum size of 0.12. In comparison to the highest biosurfactant production yield reported for Bacillus subtilis SPB1, three fold increases were obtained.
wso shell IndoXploit shell wso shell hacklink hacklink satış hacklink wso shell