Faculty of Biotechnology and Food Sciences in Nitra Journal of Microbiology, Biotechnology and Food Sciences 1338-5178 no. 1 vol. 5 2015-08-01 2015-08-01 ISOLATION OF POTENTIAL ANTIMICROBIAL COMPOUNDS FROM SALACIA OBLONGA WALL AND THEIR SYNERGISTIC EFFECT ON HUMAN PATHOGENS 7 11 EN Anjaneyulu Musini Anjaneyulu Musini Jayaram Prakash Rao Jayaram Prakash Rao Archana Giri Archana Giri Salacia oblonga Wall containing vital phytoconstituents viz. mangiferin, salacinol, kotanolol etc has been in use since long in the treatment of cancer, diabetes, inflammation and burn wounds. The antimicrobial activity of S. oblonga aerial and root parts has been studied by using ethyl acetate (EtOAc) and methanol (MeOH) solvents. Broad spectrum activity of extracts was prominent against both gram positive and gram negative human pathogens. The lowest minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) value of 0.04 and 0.08 mg/ml were observed with EtOAc root acidic extract. EtOAc extracts was analyzed by Gas chromatography and mass spectrum (GC-MS) revealing the presence of 79 compounds. The EtOAc extract was further subjected to column chromatography using silica gel and the active fraction showing the highest antibacterial activity were analyzed by liquid chromatography and mass spectrum (LC-MS). The active principles were identified as γ-sitosterol, β-Amyrin and Ursa-9(11),12-dien-3-yl acetate. Our study aims at highlighting the potential of S. oblonga extracts against various pathogen.
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