Faculty of Biotechnology and Food Sciences in Nitra Journal of Microbiology, Biotechnology and Food Sciences 1338-5178 no. 3 vol. 5 2015-12-01 2015-12-01 ANTIMICROBIAL ACTIVITY OF PULCHERRIMIN PIGMENT PRODUCED BY METSCHNIKOWIA PULCHERRIMA AGAINST VARIOUS YEAST SPECIES 282 285 EN Attila Kántor Attila Kántor Jana Hutková Jana Hutková Jana Petrová Jana Petrová Lukáš Hleba Lukáš Hleba Miroslava Kačániová Miroslava Kačániová Metschnikowia pulcherrima is common yeast on grape berries and can grow on special cultivation media with iron (e.g. FeSO4.7H2O) (max. 0.02 g/L) to produce reddish (maroon) pigment pulcherrimin. Many studies confirm that pulcherrimin has antimicrobial activity against postharvest pathogens, microscopic fungi and other yeast species. In this study, two strains of M. pulcherrima, isolated from grapes and identified by MALDI-TOF mass spectrometry, were used : VML and CBS 610NT. Sixteen yeast species: Aureobasidium (1), Candida (5), Hanseniaspora (1), Kregenvanrija (1), Pichia (2), Rhodotorula (2), Saccharomyces (1), Zygosaccharomyces (2) and Wickerhamomyces (1) were selected to test the antimicrobial capacity of these two M. pulcherrima strains. The results showed CBS 610NT had a stronger ability than VML to inhibit the tested yeasts. Among the yeast, Candida spp. and Pichia manshurica were the most sensitive to the pigment pulcherrimin.