Faculty of Biotechnology and Food Sciences in Nitra Journal of Microbiology, Biotechnology and Food Sciences 1338-5178 vol. 5 2016 2016-02-01 2016-02-01 AN EFFICIENT RECOVERY OF TRANSGENIC PLANTS FROM A TROPICAL INDIAN MAIZE INBRED LINE 335 340 EN J. Beslin Joshi J. Beslin Joshi G. Nallathambi G. Nallathambi K.K. Kumar K.K. Kumar E. Kokiladevi E. Kokiladevi L. Arul L. Arul P. Balasubramanian P. Balasubramanian D. Sudhakar D. Sudhakar Tropical Indian maize inbreds are known for their recalcitrance in tissue culture which is a prerequisite for development of transgenic lines from such cultivars. In the present study, particle bombardment-mediated transformation of a tropical Indian maize inbred line, UMI29 was attempted. Parameters that infulence transforamation efficiency such as size of embryo (1.5 mm and 2.0 mm), micro-carrier flying distance (6 cm and 9 cm) and post-bombardment resting period (2, 7 and 10 days) on transient and stable expression of transgene were investigated. The greatest transformation efficiency of 2.67% was obtained using immature embryos of 1.5 mm at 6 cm of microcarrier flying distance with a resting period of 2 days. Stable inheritance of transgenes, viz., gusA and bar was confirmed in T1 lines.
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