Faculty of Biotechnology and Food Sciences in Nitra Journal of Microbiology, Biotechnology and Food Sciences 1338-5178 vol. 5 2016 2016-04-01 2016-04-01 CHARACTERIZATION OF DIARRHEAGENIC ESCHERICHIA COLI ISOLATED FROM RAW BEEF, MUTTON, AND INTESTINES SOLD IN OUAGADOUGOU, BURKINA FASO 470 474 EN Hadiza Bawa Ibrahim Hadiza Bawa Ibrahim Gertrude Bsadjo Tchamba Gertrude Bsadjo Tchamba Touwendsida Serge Bagré Touwendsida Serge Bagré Soutongnooma Caroline Bouda Soutongnooma Caroline Bouda Alio Mahamadou Fody Alio Mahamadou Fody Assèta Kagambèga Assèta Kagambèga Isidore Juste O.Bonkoungou Isidore Juste O.Bonkoungou Fidèle Tiendrebéogo Fidèle Tiendrebéogo Cheikna Zongo Cheikna Zongo Aly Savadogo Aly Savadogo Alfred S. Traoré Alfred S. Traoré Nicolas Barro Nicolas Barro Diarrheagenic Escherichia coli are zoonotic bacteria commonly present in animal gut. The aim of our study was to determine the prevalence of diarrheagenic E. coli isolated from raw meats and intestines in open markets of Ouagadougou, Burkina Faso. A total of 450 samples were collected from beef, mutton, beef intestine and sheep intestine, in respective number 175, 175, 50 and 50. Diarrheagenic E. coli were isolated by using standard microbiological methods and then Multiplex Polymerase Chain Reaction was used for characterization. Among the pathotypes, enteropathogenic E. coli was identified by serotypage (slide agglutination). A 30% (135/450) were E. coli. 30% (40/135) of E. coli strains provided the virulence genes. 14% of Shiga toxin producing Escherichia coli, 13% of shiga toxin producing Escherichia coli-enterotoxinogenic Escherichia coli, 1% of enteroaggregative Escherichia coli, 2% of enteroinvasive Escherichia coli and 1% of enterotoxinogenic Escherichia coli. 41% (55/135) were enteropathogenic Escherichia coli belong to serotypes: (5%), O119 (3%), O127 (16%), O125 (9%), O126 (18%), O128 (5%), O114 (5%), O124 (5%), O142 (7%). This study show contamination of slaughter animal with diarrheagenic E. coli pathotypes in Burkina Faso. Precaution can take of mutation breeding level.
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