Faculty of Biotechnology and Food Sciences in Nitra Journal of Microbiology, Biotechnology and Food Sciences 1338-5178 vol. 5 2016 2016-04-01 2016-04-01 INVOLVEMENT OF EXTRACELLULAR FUNGAL ENZYMES IN BIOREMEDIATION OF TEXTILE EFFLUENT 450 455 EN Rina D. Koyani Rina D. Koyani Kishore S. Rajput Kishore S. Rajput The plentiful use and reckless discharge of textile effluent to the nature witnessed the rising of water and soil pollution. Biological remediation of these compounds is the most desirable technique to overcome the elevated environmental pollution. Present study evaluates the efficiency of a wild strain of Irpex lacteus in decolourisation and degradation of Reactive yellow FG and Reactive orange 2R. Media supplemented with different carbon/nitrogen sources and inoculum size play important role in enhancing the ability in which dextrose and aspargine boosted the process while inoculum size one-three (10 mm diameter) were more significant with solid and liquid decolourisation respectively. The ligninolytic enzyme production under Solid State Fermentation (SSF) was carried out using different lignocellulosic substrates. Among different substrates wheat straw produced highest amount (560.6 IU/ml) of manganese peroxidase. Optimization of particle size and time of incubation were also assorted to define the efficient enzyme activity; where one mm particle size and 6th day of incubation period were the most felicitous. The influence of physico-chemical factors like pH, temperature, reaction time and metal ions were assessed with respect to enzyme activity. The partial purification of crude enzymes was achieved at different percent saturations, where 40% saturated fraction yielded maximum (560.6 IU/ml) MnP activity. Molecular weight of the partially purified enzyme was 58.3 kDa. The degradation of dyes was confirmed with shift of the dominant peaks found on the FTIR graphs.
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