Faculty of Biotechnology and Food Sciences in Nitra
Journal of Microbiology, Biotechnology and Food Sciences
1338-5178
vol. 5
2016
2016-06-01
2016-06-01
AGRO-INDUSTRIAL WASTE BASED GROWTH MEDIA OPTIMIZATION FOR BIOSURFACTANT PRODUCTION BY ANEURINIBACILLUS MIGULANUS
578
583
EN
Mohamed Sellami
Mohamed Sellami
Achref Khlifi
Achref Khlifi
Fakher Frikha
Fakher Frikha
Nabil Miled
Nabil Miled
Lassad Belbahri
Lassad Belbahri
Faouzi Ben Rebah
Faouzi Ben Rebah
The present work aimed to optimize a molasses and tuna-processing by-products based new economic medium for biosurfactant (BS) production by a promising strain of Aneurinibacillus migulanus. A culture medium based on a mixture of molasses and supernatants generated from tuna by-products supplemented with oligoelements solution was optimized using the mixture design methodology. Biosurfactant (BS) production and emulsification index (E24) were evaluated. Maximal BS of 2.95 g/l was obtained with a 95:5 (v:v) mixture of molasses and tuna by-product supernatant. However, higher level of E24 (62%) was recorded with medium containing the proportion 5:95 (v:v) of molasses and tuna by-product supernatant. The predicted responses from these mixture proportions were also validated experimentally. Interestingly, oligoelements supplements were not needed to prepare the culture medium. Molasses and tuna-by-product, non-conventional substrates, can be used efficiently for BS production by A. migulanus.