Faculty of Biotechnology and Food Sciences in Nitra Journal of Microbiology, Biotechnology and Food Sciences 1338-5178 vol. 5 2016 2016-06-01 2016-06-01 PURIFICATION AND CHARACTERIZATION OF SOLVENT STABLE LIPASE FROM A SOLVENT TOLERANT STRAIN OF GEOBACILLUS STEAROTHERMOPHILUS PS 11 602 605 EN Payel Sarkar Payel Sarkar Khusboo Lepcha Khusboo Lepcha Shilpi Ghosh Shilpi Ghosh An extracellular organic solvent stable lipase produced by solvent tolerant strain of Geobacillus stearothermophilus PS11 was purified and characterised. The overall purification was 8.04 fold with a yield of 22.6%. The molecular weight of purified lipase was approximately 27.5 kDa. The purified lipase activity was stable (745 EU/ml at 72h incubation) in presence of toluene, benzene, propanol, methanol etc. The enzyme activity was maximum (764 EU/ml) when assayed under optimum temperature and pH of 50⁰C and 10.0, respectively. The enzyme showed stability at a wide range of temperature from 10⁰C to 60⁰C. This solvent stable lipase can be a novel tool for biodiesel industry.
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