Faculty of Biotechnology and Food Sciences in Nitra Journal of Microbiology, Biotechnology and Food Sciences 1338-5178 vol. 5 2016 2016-06-01 2016-06-01 COST-EFFECTIVE PRODUCTION OF THE BIO-PLASTIC POLY-β-HYDROXYBUTYRATE USING ACINETOBACTER BAUMANNII ISOLATE P39 552 556 EN Noha Salah Elsayed Noha Salah Elsayed Khaled Mohamed Aboshanab Khaled Mohamed Aboshanab Mohammad Mohammad Aboulwafa Mohammad Mohammad Aboulwafa Nadia Abdelhaleem Hassouna Nadia Abdelhaleem Hassouna Being biodegradable and biocompatible natural polymer, poly-β-hydroxybutyrate (PHB) drew the attention of scientists to substitute synthetic plastics in our daily lives. However, its industrial production is hampered by its high cost. In this study, an extensive screening program was done to isolate bacteria with high PHB productivity from agricultural fields and develop a cost-effective PHB production. A promising bacterial isolate Acinetobacter baumannii P39 was recovered and identified using 16S ribosomal gene sequencing. It produced 24% PHB per dry weight after 48 h. Several experiments were conducted to optimize the composition of the culture medium and environmental factors for the selected isolate. Results revealed that 60% aeration, 28°C incubation temperature and initial pH 7.5 showed the highest productivity. Besides, 0.7% corn oil and 0.1 g/L peptone were the best carbon and nitrogen sources, respectively. Substituting glucose with corn oil led to a 23% reduction in total input cost and an estimate price for 1kg PHB is 20.5 L.E. Strain improvement by UV mutation succeeded in improving PHB production by two fold in the selected mutant P39M2. Finally, this study valorizes usage of Acinetobacter isolate in PHB production in addition to solving the critical problem of high cost of production.
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