Faculty of Biotechnology and Food Sciences in Nitra Journal of Microbiology, Biotechnology and Food Sciences 1338-5178 vol.6 2016 2016-10-03 2016-10-03 IN VITRO EFFICACY OF BRACKET FUNGI FOR THEIR POTENTIAL ANTIMICROBIAL ACTIVITY 818 822 EN Vipin Parkash Vipin Parkash Anita Sharma Anita Sharma This study was conceptualized to reveal the anti-microbial potential of bracket fungi, viz. - Ganoderma lucidum (Curtis) Karst. and Polyporus officinalis (syn. Laricifomes officinalis) (Vill.) Fr. extracts through in vitro approach. The aqueous and ethanolic extracts were assessed against four phytopathogenic fungi (Alternaria solani, Curvularia lunata, Aspergillus te¬rreus and Fusarium oxysporum) along with bacteria (Escherichia coli and Bacillus subtilis). Ethanolic extract of G. lucidum had significant growth inhibition effect against A. solani, C. lunata, A. te¬rreus and F. oxysporum at 500ppm and 1000ppm concentrations. On the other hand, aqueous extract showed complete inhibition at 1000ppm and 500ppm concentrations against A. terreus and F. oxysporum. Ethanolic extract of P. officinalis, complete fungal growth inhibition was observed against A. solani and A. terreus at 1000ppm concentration while for C. lunata and F. oxysporum, complete inhibition was observed at 1000ppm and 250ppm concentration. Similarly, the aqueous extract of same bracket fungus, showed maximum inhibition of A. solani, A. terreus and F. oxysporum at 1000ppm concentration but C. lunata had maximum inhibition at 250ppm concentration. The antibacterial action of ethanolic extract of G. lucidum was observed against E. coli having inhibitory zone (0.09 mm) at 1000 ppm concentration and B. subtilis had inhibitory zone (0.05mm) at 250ppm concentration. But no inhibitory zones were observed in E. coli and B. subtilis with aqueous extract of G. lucidum. Whereas, the ethanolic extract of P. officinalis showed a maximum inhibitory zone (10 mm) at 1000 ppm concentration for E. coli and a remarkable inhibitory zone (0.2 mm) at 250 ppm concentration for B. subtilis. While an inhibitory zone (0.3 mm) was observed in E. coli at 250 ppm concentration of aqueous extract, but no inhibitory zone was observed for B. subtilis at any concentration of the aqueous extract of P. officinalis. Based on the study, it can be concluded that G. lucidum and P. officinalis are having considerable potential as anti-fungal and anti-bacterial action, respectively.
wso shell IndoXploit shell wso shell hacklink hacklink satış hacklink wso shell