Faculty of Biotechnology and Food Sciences in Nitra Journal of Microbiology, Biotechnology and Food Sciences 1338-5178 vol.6 2016 2016-10-03 2016-10-03 LACCASE PRODUCING STREPTOMYCES BIKINIENSIS CSC12 ISOLATED FROM COMPOST 794 798 EN Devi P. Devi P. Sujatha Kandasamy Sujatha Kandasamy Chendrayan K Chendrayan K Sivakumar Uthandi Sivakumar Uthandi Lignin degradation by actinobacteria is more attractive than well known white rot fungal system by the fact of their higher survival rate, sporulating ability, economic production of useful chemicals rather than evolution of CO2 as in white-rot fungi. One of the important enzymes in ligninolysis is laccase, which oxidize a broad range of substrates, preferably phenolic and non-phenolic compounds. Among the laccase producing microbes in nature, white rot fungi have been characterized much, very little is known about the laccase producing actinobacteria. The present investigation aims to isolate and screen active laccase producing actinobacteria from diverse environments. Fifteen isolates of actinobacteria were isolated from various organic substrates viz., coir compost, groundnut shell compost, fully and partially decomposed municipal solid waste and identified based on molecular characterization. With initial screening using ρ-anisidin and Poly-R 478, seven actinobacterial isolates had more efficient ligninolytic activity than others. Of which, isolate CSC12 secreted highest laccase and was identified as Streptomyces bikiniensis CSC12. Laccase from S.bikiniensis CSC12 was partially purified as monomer having molecular mass ~69 kDa based on SDS-PAGE and had pH and temperature optima of 6 to 7 and 50 to 60 °C respectively.
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