Faculty of Biotechnology and Food Sciences in Nitra Journal of Microbiology, Biotechnology and Food Sciences 1338-5178 vol. 6 2016/17 2016-12-01 2016-12-01 DIESEL BIOTREATMENT COMPETENCE OF INDIGENOUS METHYLPARATHION DEGRADING BACTERIAL STRAIN OF PSEUDOMONAS AERUGINOSA DOU 878 885 EN Krishnaswamy Rathinam Usharani Krishnaswamy Rathinam Usharani Perumalsamy Lakshmanaperumalsamy Perumalsamy Lakshmanaperumalsamy Pseudomonas aeruginosa methylparathion degrading bacterial strain previously isolated and identified in our lab was subjected to confirm their ability to diesel oil degradation in synthetic wastewater containing diesel oil (10 % v/v). They are cultured in modified mineral salts agar medium with diesel oil as the sole source of carbon. They were able to grow and distinguish in 7 days of incubation. Biosurfactant was produced in the medium and its effect was detected using emulsification activity, growth estimation and gravimetric analysis as an indicator for this process. Factors affecting hydrocarbon utilization by this isolate were pH, temperature, salt concentration and agitation along with glucose, peptone, phosphate and nitrate concentrations. Gravimetric and FTIR analysis showed that the strain was able to degrade complex hydrocarbon chains to simple ones. The optimum conditions of various factors showed that, neutral pH, with temperature of 320C, and agitation rate of 150 rpm gave the optimum conditions to accelerate diesel oil degradations, while additional nutrients of glucose (1 mg/l), peptone (5 mg/l), sodium nitrate (2 mg/l), phosphate (7 mg/l) and sodium chloride (3 mg/l) enhanced optimum effect on the bioprocess. The bacterial strain of indigenous pure monoculture having the degradative potential of xenobiotic compounds of various pollutants includes diesel oil as well as methylparathion pesticide practically. The main conclusion is that Pseudomonas aeruginosa dou-1 strain can be able to degrade complex hydrocarbons and make it an ideal applicant in bioremediation.
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