Faculty of Biotechnology and Food Sciences in Nitra Journal of Microbiology, Biotechnology and Food Sciences 1338-5178 vol. 6 2017 2017-02-01 2017-02-01 ISOLATION AND IDENTIFICATION OF Elizabethkingia meningoseptica FROM DISEASED AFRICAN CATFISH Clarias gariepinus 1070 1076 EN Laith A.A Laith A.A Mazlan A.G Mazlan A.G Ambak M.A Ambak M.A Jabar A Jabar A Najiah M. Najiah M. Two isolates of Elizabethkingia meningoseptica were successfully isolated from kidney and skin tissue of diseased African Catfish (Clarias gariepinus) in Malaysia. The percentage similarity of both physical and biochemical characteristics for the isolates from kidney (K1) and skin samples (S1) as determined by BBL-Crystal and API 20E were 99 % and 95.4%, respectively. Furthermore, both isolates were identified via 16S rRNA gene sequences and showed more than 97% homology to sequences deposited in GenBank. The API ZYM results were analogous for both strains, with only minor quantitative variations. However, the isolates from kidney sample (K1) showed higher levels of enzymatic activity reaction towards esterase lipase and leucine arylamidase. Moreover, the enzymatic activity of α-galactosidase was detected at low level in kidney isolate and absence in skin isolate.
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