Faculty of Biotechnology and Food Sciences in Nitra Journal of Microbiology, Biotechnology and Food Sciences 1338-5178 no. 5 vol. 6 2017-04-03 2017-04-03 ANTIMICROBIAL ACTIVITY of SPIRULINA PLATENSIS AGAINST AQUATIC BACTERIAL ISOLATES 1203 1208 EN Wagih Abd El-Fattah Elshouny Wagih Abd El-Fattah Elshouny Mostafa Mohammed El-Sheekh Mostafa Mohammed El-Sheekh Shawky Zaki Sabae Shawky Zaki Sabae Maha Abdelfattah Khalil Maha Abdelfattah Khalil Hanaa Mohamed Badr Hanaa Mohamed Badr Aquatic organisms are a rich source of novel and bioactive compounds. Cyanobacteria and microalgae being a rich source of bioactive compounds have recently found immense application in human and animal medicine. The present study was attempted to find out the effect of the various extracts of Spirulina platensis, Chlorella vulgaris, Saragassum wightii and Saragassum latifolium using different solvents (methanol, ethanol, ethyl acetate and chloroform) as antimicrobial agents against five bacterial pathogens; S. aureus, E. coli, P. aeruginosa, Salmonella sp, Shigella sp. Results indicate that among the various extracts used, methanol extracts of tested cyanobacterial and algal species appeared to be the most effective ones showing maximum antibacterial activity against the selected bacterial pathogens. Spirulina platensis appeared to be the most effective against all the pathogens studied. The antibacterial substance was purified using column chromatography. The nature of the purified active fractions was detected using different chemical analyses (UV, FT-IR, 1H NMR and GC-MS) which indicated that it is an aliphatic compound and has different active groups (-OH,-C=O,-CH2 and –CH3). The results of this investigation proved that the tested cyanobacterium could be a good source for the production of promising antimicrobial agents.
film izle