Faculty of Biotechnology and Food Sciences in Nitra Journal of Microbiology, Biotechnology and Food Sciences 1338-5178 vol. 6 2017 2017-06-01 2017-06-01 PREPARATION OF PLANT VECTOR CONSTRUCT CONTAINING DEHYDRIN GENE At2g21490 1261 1263 EN Mária Zimová Mária Zimová Eva Boszorádová Eva Boszorádová Zuzana Gregorová Zuzana Gregorová Ildikó Matušíková Ildikó Matušíková Jana Moravčíková Jana Moravčíková This work is focused on preparation of a plant transformation vector pDH2 containing the expression unit of the dehydrin gene At2g21490 under a control of the constitutive dCaMV 35S promoter and the selectable marker neomycin phosphotransferase gene controlled by the nos promoter. The gene At2g21490 is classified as a dehydrin of the type K2SY2. The gene was isolated from Arabidopsis thaliana by PCR approach. The plasmid pDH2 was transformed into the strain Agrobacterium tumefaciens LBA 4404. The stability of the plasmid pDH2 in Agrobacterium has been verified by restriction analysis after re-transformation of the pDH2 into Escherichia coli.
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