Faculty of Biotechnology and Food Sciences in Nitra Journal of Microbiology, Biotechnology and Food Sciences 1338-5178 no. 3 vol. 7 2017-12-01 2017-12-01 BIOCHEMICAL CHARACTERIZATION OF ANTI-METHICILLIN RESISTANT S. aureus PROTEIN (P-80) FROM MARINE Pseudoalteromonas 294 298 EN Jorge Bautista Secun Jorge Bautista Secun Alejandra Hernández Santoyo Alejandra Hernández Santoyo Víctor Monteón Padilla Víctor Monteón Padilla Oscar Hernández Vázquez Oscar Hernández Vázquez Ruth López Alcántara Ruth López Alcántara The marine Pseudoalteromonas are recognized as major active metabolite producers against different biological targets. These compounds include cyclic peptides, toxic proteins or enzymes, among others. In previous studies, we isolated a protein named P-80 from Pseudoalteromonas sp with antibiotic activity against S. aureus, and proteolytic action. In the present work, we studied properties such as structure and the function of P-80. We performed different assays, including MALDI-TOF mass spectrometry, antibacterial activity by the standard disc diffusion, pH and temperature stability, cytotoxicity in vitro and finally the effect of metal ions and protease inhibitors on antimicrobial activity. The purified P-80 showed a molecular mass of 78.5 kDa as determined by MALDI-TOF mass spectrometry. It was highly active over pH range of 7.0 to 9.2, and remained stable over a pH range of 7.0 to 8.0 although its activity was reduced at pH 9.2 (only 55% was retained). This protein showed quite a good antibacterial activity over a broad temperature range (> 65% of activity is retained between -20 and 70 ºC). The inhibitor tested such as EDTA, EGTA, PMSF, DTT and TLCK (at 5 mM) had no effect on the antibacterial activity. Whereas Mn2+ ions enhanced the antibiotic activity but Zn2+, Fe2+, Mg2+, Ca2+, Cu2+ and Co2+ ions had no effect. In conclusion, the P-80 protein remained 55% and 65 % of its antimicrobial activity against Staphylococcus aureus at pH 9.0 and 70 ºC respectively, suggesting high stability compared to other marine antibacterial proteins indicating intrinsic features that could enable commercial applications.
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