Faculty of Biotechnology and Food Sciences in Nitra
Journal of Microbiology, Biotechnology and Food Sciences
1338-5178
no. 3
vol. 7
2017-12-01
2017-12-01
DETECTION OF PENICLLIUM OXALICUM IN GRAPES WITH A LOOP-MEDIATED ISOTHERMAL AMPLIFICATION (LAMP) ASSAY
265
270
EN
Elisabeth I. Vogt
Elisabeth I. Vogt
Veronika M. Kupfer
Veronika M. Kupfer
Julia D. Bechtner
Julia D. Bechtner
Lisa M. Frisch
Lisa M. Frisch
Rudi F. Vogel
Rudi F. Vogel
Ludwig Niessen
Ludwig Niessen
P. oxalicum is known as plant pathogen, mycotoxin producer and was suggested to be involved in the induction of champagne gushing. Early detection of the fungus enforces decision making in the wine industry as to how grapes will be further processed. A loop-mediated isothermal amplification (LAMP) assay for P. oxalicum was established with a detection limit of 100 pg genomic DNA per reaction. The assay was highly specific for P. oxalicum DNA. The assay was applied for the detection of the fungus on artificially infected grapes using surface washings as target for the LAMP reaction. Screening of grape samples from different European countries revealed the (rare) presence of P. oxalicum on grapes harvested in Italy and Portugal. We suggest the developed LAMP assay as a promising new tool for the rapid, robust, highly specific and sensitive as well as cost efficient detection of P. oxalicum on grapes used in the wine and champagne industry.