February 2014, vol. 3, special issue 2 (Biotechnology)
Marcela Capcarová, Adriana Kolesárová, Alexander V. Sirotkin
Biotechnology of Biotechnology
The aim of this study was to determine the activity of superoxide dismutase (SOD) in rat ovarian fragments cultured in vitro after molybdenum (Mo) and silver (Ag) administrations. Ovarian fragments were incubated with Mo (ammonium molybdate, (NH4)6.Mo7O24.4H2O) and Ag (silver nitrate, AgNO3) as follows: 0.13 mg.ml-1 in E1 group, 0.17 mg.ml-1 in E2, 0.33 mg.ml-1 in E3, 0.5 mg.ml-1 in E4, and 1.0 mg.ml-1 in E5 for 24 hours. The group without any addition served as the control. Activity of SOD in ovarian fragments significantly decreased in all experimental groups when compared to the control suggesting uncontrolled overproduction of reactive oxygen species (ROS) and failure in antioxidant defence system. Trace elements can adversely affect animal reproductive system and its functions, through either direct or indirect effects on oxidative stress induction.