February 2014, vol. 3, special issue 2 (Biotechnology)
Zdenka Gálová, Eva Pálenčárová, Milan Chňapek, Želmira Balažová
Biotechnology of Biotechnology
Our work was focus on amaranth (Amaranthus sp.) cv. Plaisman and buckwheat (Fagopyrum Mill.), cv. Pyra proteome, which was analysed by 2-D electrophoresis. We found similarity between the chemical properties proteins of pseudocereals amaranth and buckwheat. Image analysis showed a higher number of spots on 2-DE map of buckwheat in comparison to amaranth. Some similarities were in protein spots at approximately 21,000 Da, pI 7 and strip of protein spots in range of pI 8-10, 21,000 Da. The buckwheat 2-DE map shows spots of protein with higher intensity in the region ranging from 30-45,000 Da, pI 5-6 as well as highly abundant protein spots from visible at 36-40,000 Da, pI 8-9. Protein maps showed that the pseudocereals do not content storage proteins, which indicates that they are suitable as a replacement for cereals for people with celiac disease.
Amaranth, buckwheat , 2-DE protein maps, celiac disease