February 2014, vol. 3, special issue 2 (Biotechnology)
Daniela Chmelová, Miroslav Ondrejovič
Biotechnology of Biotechnology
In this work, the influence of selected inorganic ions (Cu2+ and Mn2+) and aromatic amino acids (tryptophan and tyrosine) on laccase production by white-rot fungus Ceriporiopsis subvermispora was investigated. This aim was realized by monitoring laccase production during cultivation of C. subvermispora. Secondary, we been evaluated glucose concentration in medium and biomass growth after cultivation. Extracellular laccase formation can stimulated by the addition of Cu2+ (3.0 mmol/L). The higher laccase activity reached maximum at 7th day (63 U/L), equivalent to 3.7-fold higher than the laccase production without copper (17.2 U/L). Higher concentration of copper ions had a negative effect on laccase production. The addition of copper ions inhibited the biomass growth. Mn2+ ions similarly stimulated laccase activity (3.0 and 7.0 mmol/L; 79.6 and 63.8 U/L, respectively) and maximum activities were reached at 6th day. Manganese ions also stimulated fungal biomass of C. subvermispora. The addition of aromatic amino acids did not cause an increase laccase production. The highest laccase production was observed in cultivation media without aromatic amino acids (16.0 U/L) at 8th day.