February, 2014, vol. 3, special issue 1 (Microbiology)
Miroslava Kačániová, Marcela Capcarová, Katarína Zbyňovská, Peter Haščík, Martin Melen, Jana Petrová, Ivana Charousová, Lukáš Hleba
Microbiology of Microbiology
The aim of this study was to compare the microbial species in caecum microflora of rabbit in control group against experimental group where epicatechin and patulin were applied. It is first study about caecum microflora study after epicatechin and patulin toxin application. In this study classical method was used for enumeration of bacteria . Enterococci were counted on Slanetz-Bartley agar (Biolife, Italy) and incubated at 37 °C for 48-72 hours, lactobacilli were counted on MRS Lactobacillus agar (Biolife, Italy) and incubated at 37 °C for 48-72 hours and coliforms bacteria were counted on MacConkey agar (Biolife, Italy) and incubated at 37 °C for 24-48 hours. In our study the number of coliforms bacteria ranged between 3.78 to 4.45 log cfu.g-1, lactobacilli ranged between 3.85 to 5.00 log cfu.g-1 and enterococci ranged between 3.48 to 4.48 log cfu.g-1.