February 2014, vol. 3, special issue 2 (Biotechnology)
Anna Latacz, Krystyna Pierzchała-Koziec, Joanna Zubel-Łojek, Ewa Ocłoń
Biotechnology of Biotechnology
In this in vitro study the effects of rapamycin – mTOR kinase inhibitor, on the activity of kinase complexes componenents Rictor and Raptor, and on astrocytes activity were investigated. Astrocytes were incubated for 24 hours in various glucose conditions: normoglycemia (CONTROL : 4.5 g glucose.L-1), high glucose (H1: 9 g glucose.L-1 ), very high glucose (H2:13.5g glucose.L-1). Two concentrations of rapamycin (1nM and 10nM) were added to the culture medium. The activity of kinase complexes was evaluated by quantitative PCR measurements of mRNA expressions for Raptor, Rictor and markers of astrocytes activity – GFAP. Significant differences have been detected between control and experimental groups. Observed data suggested that rapamycin reduced mTOR kinase activity in control group and increased activity of astrocytes in elevated glucose conditions in vitro.