February 2014, vol. 3, special issue 2 (Biotechnology)
Aleš Pavlík, Petr Sláma, Zdeněk Havlíček, Radek Filipčík
Biotechnology of Biotechnology
The aim of present study was to test hypothesis, that the leptin gene single nucleotide polymorphism (C/T) giving missense mutation (Arg25Cys) has an effect on concentration of blood serum total cholesterol, beta-hydroxybutyrate and urea in cattle. The experiment were performed in 58 Czech Pied bulls at 240 ± 9 days of age, which were divided in three experimental groups depending on different leptin genotypes (CC, n=28; CT, n=21; TT, n=9). Resulting genotypes in the exon 2 were CC (48.3%), CT (36.2%), and TT (15.5%). There were no differences in serum total cholesterol, urea, beta-hydroxybutyrate concentrations among the genotypes. Based on our results we may assume that analysed SNP of leptin gene have no effect on nutritional status and energy balance in fattened cattle.
beef cattle, leptin gene, blood serum, energy balance