February 2014, vol. 3, special issue 2 (Biotechnology)
Tomáš Slanina, Michal Miškeje, Ida Petrovičová, Peter Massányi
Biotechnology of Biotechnology
The aim of this study was to determine the effect of selected concentration of caffeine on the turkey spermatozoa motility during in vitro cultivation at 41°C. Semen samples diluted with physiological solution were used as the control sample – KT and experimental samples constituted semen diluted with caffeine solution - 1.25 mg.ml-1 – ET; 0.625 mg.ml-1 – FT; 0.313 mg.ml-1 – GT; 0.156 mg.ml-1 – HT; 0.078 mg.ml-1 – IT. Motility parameters were analyzed at six time periods: 0, 1, 2, 3, 4 and 5 hours. Measurement was evaluated by the Computer Assisted Semen Analyser. The highest spermatozoa motility of 70.28 % was detected in the sample ET at the beginning of cultivation. Considerable decrease values were observed after 1 hours of cultivation in all samples (KT – 31.15; ET – 27.15; FT – 15.74; GT – 15.98; HT – 18.78; IT – 15.81%). In all experimental samples higher percentage of motility in comparison to the control at the time 1 was detected. However these differences were not significant. Spermatozoa progressive motility showed similar tendency as spermatozoa motility. Non-significant differences were found in all time periods of in vitro cultivation between control sample and samples with the addition of caffeine. The results of our study suggest that the addition of caffeine has no significant positive effect on the motility parameters of turkey spermatozoa during in vitro cultivation at 41°C.
caffeine, turkey, spermatozoa, motility, CASA