PLANT TRANSFORMATION VECTORS AND THEIR STABILITY IN AGROBACTERIUM TUMEFACIENS

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Special issue 1 – part A 2013, vol. 2, special issue 1 part A
pages: 1559-1568
Article type: Biotechnology of Biotechnology
Abstract: The stability of the plant transformation vectors pTS2, pEV2 and pEV8 was tested in two Agrobacterium tumefaciens strains LBA 4404 and C58C1 that differed in chromosomal background. The T-DNAs of all binary vectors contained the β-glucuronidase reporter and the neomycin phosphotransferase selectable marker genes. In addition, the plasmids pEV2 and pEV8 contained the Cre/loxP recombination system. The binary vectors were transformed into A. tumefaciens strains and their stability was evaluated by restriction analyses after retransformation of plasmids from A. tumefaciens into E. coli. Our results showed that plasmids pTS2 and pEV2 were stable in both bacterial strains while the pEV8 exhibited instability in A. tunmefaciens LBA 4404.
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