SCREENING OF COMMON FLAX FAD GENES BY PCR

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Special issue 1 – part B 2013, vol. 2, special issue 1 part B
pages: 1665-1676
Article type: Biotechnology of Biotechnology
Abstract: Currently, flax (Linum usitatissimum L.) is an important crop from commercial and economical aspects. In the spotlight is the linseed oil as a source of α-linolenic acid. The aim of presented study was to analyse fatty acid desaturase (FAD) genes in flax. Several genotypes of flax (Hohenheim, La Plata 1938, Redwing USA and Escalina) were used. The primers described by Vrinten et al. (2005) were used for PCR amplification reactions. Two FAD3 genes, LuFAD3A and LuFAD3B, were identified in a genome of flax. Subsequently the nucleotide sequences between origins and genotypes of flax FAD genes were compared. Primarily were used the nucleotide sequences of FAD2 and FAD3C genes available in NCBI database. Differences were found using BLAST program in nucleotide sequences of FAD genes and the specific primers were designed to amplify a specific target sequences in a genome of flax. These primers were used in PCR amplification reactions to identification of FAD2 and FAD3C genes. The PCR products were separated by electrophoresis on agarose gel.
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