Special issue 1 – part B 2013, vol. 2, special issue 1 part B
Jaromír Vašíček, Peter Chrenek
Biotechnology of Biotechnology
The objective of our study was to evaluate the influence of different storage temperature on rooster sperm motility. Semen was collected once a week from Lohmann Light breeder males into prepared sterile tube. The heterospermic pool was diluted at the ratio of 1:100 in a commercial avian extender, divided into two aliquots and incubated at 8°C or 37°C. The quality of semen samples were evaluated using CASA system (Sperm Vision™) after 30, 60 and 120 min of incubation. We observed significantly (P<0.05) the highest progressive motility of rooster sperm after 60 min of spermatozoa incubation at 8°C, that was also significantly (P<0.05) higher in comparison to spermatozoa incubated for 60 min at 37°C. Basing on the observed results, we hypothesized that law temperatures (about 8°C) would be better for long-term storage of rooster spermatozoa. Further experiments with different semen diluents and storage temperatures and intervals are required in order to prove our hypothesis.
rooster, spermatozoa, temperature, storage interval, CASA