Special issue 1 – part B 2013, vol. 2, special issue 1 part B
Marcela Capcarová, Adriana Kolesárová, Alexander V. Sirotkin
Biotechnology of Biotechnology
The aim of this study was to determine the activity of superoxide dismutase (SOD), total antioxidant status (TAS) and expression of heat shock protein 70 (Hsp70) of porcine ovarian granulosa cells cultured in vitro after cobalt (Co) administrations. Ovarian granulosa cells were incubated with cobalt sulphate administrations as follows: group E1 (0.09 mg.ml-1), group E2 (0.13 mg.ml-1), group E3 (0.17 mg.ml-1), group E4 (0.33 mg.ml-1), group E5 (0.5
mg.ml-1) and the control group without any additions for 18 h. Co administration developed stress reaction and promoted accumulation of Hsp70 what resulted in increasing activity of SOD. TAS of granulosa cells increased with higher doses of Co whereas low doses had no effect on this parameter. Trace elements can adversely affect animal female reproductive system and its functions, through either direct or indirect effects on oxidative stress induction.
Granulosa cells, cobalt, SOD, TAS, Hsp70