Zofia Goc, Katarzyna Kilian, Grzegorz Formicki, Robert Stawarz, Agnieszka Greń, Renata Muchacka
Biotechnology of Biotechnology
The aim of this study was determination of the concentration of calcium and magnesium in human milk (n=150) taken from nursing women who lived permanently in Malopolska district (South Poland). Milk samples were classified into groups taking into account women’ age and parity. According to the various age of the women milk samples were segregated into three groups: 20-25 years old, 26-31 years old and 32-37 years old. Included parity milk samples were spitted into two groups: the first group consisted of women who were primiparous (1 baby), the second group included women who were multiparous
(≥2 babies). Milk samples were taken between the 7th and 14th day of the postpartum in each age group. The samples were taken by manual expression every morning. The analyses of metals were done by flame atomic absorption spectrophotometry (FAAS), after all samples had been collected. The mean concentration of cooper in milk taken from women of first age group (20-25 years old) was 209.44±15.10 mg/L, in second group (26-31 years old) was 238.65±13.34 mg/L and in the oldest group (32-37 years old) was 261.44±17.16 mg/L. The mean concentration of magnesium in the same group of age was 42.12±3.793 mg/L, 47.51±2.728 mg/L and 45.43±3.840 mg/L, respectively. The mean concentration of calcium in milk taken from primiparous was 223.17±10.50mg/L, in multiparous was 266.37±16.20 mg/L. Whereas, the mean concentration of magnesium in transitional milk taken from the same group was 44.12±2.58 mg/L and 47.412±3.16 mg/L, respectively. Statistical analyses showed that there were statistically significant differences between calcium level in milk taken from the youngest group and the concentration of this metal in milk from the oldest group (p=0.032). Also, when comparing women’s parity, significant differences were found in the concentration of calcium (p=0.022). However, the differences in magnesium levels between tested groups were no statistically significant. Additionally, r Pearson correlation coefficient showed positive correlation between analyzed metals (Mg/Ca: r2=0.589; p=0.000). These observations suggest that mothers’ age and parity have influence on calcium concentration in milk but did not impact on magnesium level in breast milk.