The aim of our study was to work the antimicrobial and antioxidant activities of Citrus paradisi peel, Citrus reticulata peel, Juniperus communis fruit, Eucalyptus globulus leaf, and Cananga odorata flower essential oils. Essential oil samples were analysed by GC chromatography coupled with FID detector (Agilent 6890N). The plant essential oils were tested regarding their antioxidant activity and antimicrobial activity against 13 species of Gram positive (G+) and Gram negative (G-) bacteria isolated from human semen and identified by MALDI-TOF MS Biotyper. Isolated G+ and G- were tested for antibiotics resistance Among tested bacteria, the lowest antibiotic susceptibility for Corynebacterium species were observed for ciprofloxacin in three cases, for gentamycin in three cases, for vancomycin in two cases and for tetracycline in five cases. Antioxidant activities tested using DPPH method reveals that Cannaga odorata had the higher antioxidant activity and the lowest was found at J. communis essential oil. Antimicrobial activities showed that all the essential oils inhibited on the entire 13 species of Gram positive (G+) and Gram negative (G-) bacteria being used. However, C. odorata have the best antimicrobial effect against Corynebacteria. Good antimicrobial activity of C. paradise was found against Corynebacteria also. Strong antibacterial activity of C. odorata against E. coli, E. faecium, P. aglomerans, P. fulva and S. agalactiae were found.