The polysaccharides biosynthesized by the microorganisms had emerged as an important class of bioactive compound from the last two decades. In the present study, an attempt was taken to evaluate the in vitro antioxidant activity of EPS produced by Micrococcus luteus SNIST-CM02. The scavenging assays involving 1, 1-diphenyl-2-picrylhydrazyl (DPPH), superoxide radicals, hydroxyl radicals, chelating ability and inhibition effect of lipid peroxidation, for the biosynthesized EPS were studied in order to evaluate the in vitro antioxidant activity. The EC50 value of EPS for DPPH, superoxide radicals and hydroxyl radicals was found to be 8.11 mg/mL, 8.00 mg/mL and 10.25 mg/mL respectively. The EPS had a lower inhibition effect of lipid peroxidation than that of ascorbic acid and butylated hydroxytoluene. At a concentration of 12.0 mg/mL, the inhibition was only 70.00%. These results suggest that the EPS synthesized by Micrococcus luteus SNIST- CM02 can be used as a good potential antioxidant and can be added to different types of food as a novel antioxidant.
Antioxidant; DPPH; Scavenging assay; Micrococcus luteus; EPS; EC50 Value