The aim of this work was to analyze the effect of intravaginal administration of commercial preparation Supergestran (GnRH) as a substitute for its intramuscular administration to induce ovulation of inseminated does. GnRH analysis of reproductive performance is divided into experiments focused on fertility parameters (conception ratio, number of live and dead born of pups and birth weight of pups), and monitoring sperm-ovule interaction. The experiments were carried out under conditions of 3 rabbit breeds (experimental breed, production breed 1 and production breed 2). Intravaginal administration of GnRH at a concentration of 0.3 ml of commercially available Supergestran to 0.5 ml of ID (insemination dose) completely replaces intramuscular administration, which is normally used in rabbit breeding at AI (artificial insemination). Experiments on nulliparous females, flushing out of zygotes, showed little improvement in the conception ratio in favor of the group of inseminated females with the treated ID (77.66%) versus the group of females inseminated with control ID (74.55%) (no statistically significant differences). Conception ratios obtained from three different breeds (on farm P1, P2, E) demonstrate only minor differences between the monitored groups of females (without statistically significant differences).