In this study, it was aimed to investigate the effect of exopolysaccharide produced by 2 novel probiotic strains (Lactobacillus plantarum GA06 and GA11), isolated from local handmade cheese, on cholesterol mechanism and bile deconjugation. These two strains were thought to play an important role in cholesterol removal and may contribute to intestinal microbiota positively. The bacterial 16S rRNA gene sequencing analysis was used for molecular identification of the strains. Exopolysaccharide production of the strains was identified by the phenol-sulphuric acid method whereas cholesterol removal and glycocholate deconjugation activity were evaluated via spectrophotometric methods. The exopolysaccharide production of L. plantarum GA11 and GA06 was determined as 96.5 and 32.5 mg/L, and the cholesterol removal amounts were found as 36.7% and 28.6% respectively. Glycocholate deconjugation activity of GA11 was detected higher than of GA06. A statistically significant difference was observed between the EPS production capacity of the strains and the removal of cholesterol (p<0.05). And also, a statistically significant correlation was found both between the EPS production capacity- cholesterol removal and between EPS production capacity-glycocholate deconjugation. According to the results of our study, L. plantarum GA11 strain appears to be a good and novel probiotic strain that has high probiotic potential. Further studies can be confirmed to determine specific more crucial health benefits.