Adriana Kolesárová*, Marcela Capcarová, Nora Maruniaková, Zuzana Baková, Róbert Toman, Sushmita Nath, Alexander V. Sirotkin
Biotechnology of Biotechnology
Fusarium toxins stable natural toxins produced by Fusarium species can appear in the food chain. The aim of this in vitro study was to examine: 1. the effect of deoxynivalenol (DON) treatment on the ability of rat ovarian cells to release insulin-like growth factor I (IGF-I) and 2. a possible signaling pathway of cell apoptosis through selected markers (Bcl-2, Bax and caspase-3) induced by this natural toxin. Rat ovarian fragments were incubated with DON for 24h: 10, 100 and 1000 ng.ml-1, while the control group received no DON. The secretion of IGF–I was determined by RIA and expression of antiapoptotic (Bcl-2) and proapoptotic (Bax and caspase-3) proteins by Western-blotting analysis. IGF-I release by rat ovarian cells after DON addition was not significantly (P>0.05) stimulated (at the dose 10 ng.ml-1) and inhibited (at the doses 100 and 1000 ng.ml-1) by the toxin. The impact of DON on the expression of Bcl-2 (26 kDa), Bax (23 kDa) and caspase-3 (34 kDa) was found. Bcl-2 expression was decreased and Bax and caspase-3 increased by DON treatment. In conclusion, our results suggest a direct effect of DON on (1) ovarian functions, (2) mechanisms of proliferation and apoptosis in rat ovarian cells through intracellular regulators: Bcl-2, Bax and caspase-3.
Deoxynivalenol, IGF–I, apoptosis, ovary, rat