Abstract: In this work we investigated the effect of three types of fat substances based on the use of 5% packed fat (PF) as saturated fat (SF) for control group and 2.5%PF+2.5% rapeseed for treatment1. 2% PF+2.5% sunflower oil as unsaturated fat (USF) and also mixing between them with different level proportion in the diet of broiler (Ross-308) chickens. Lower cholesterol levels in the blood were observed in groups T1 for male and female (3.83, 3.37 Mmol.l-1) respectively compared to other groups. The results show that lowest group for triglyceride for female was in group T2 (0.33 Mmol.l-1) and for male was in Cgroup (0.34Mmol.l-1). High level of total protein in blood for female was in group T3 (37.04g.l-1) and for male was in group T2 (48.35g.l-1). Differences were significant (P≤0.01).