Abstract: Hydrolyzed Bermuda grass (Cyanidon dactylon) and Jambul seed (Syzygium cumini), were used as carbon sources for the production of Polyhydroxyalkanoate (PHA) from soil microbial isolates. The efficiency of selected isolate for PHA production utilizing the hydrolyzed substrate as carbon source was compared with Ralstonia eutropha (reference strain) using the same production medium. The best isolate SP-Y1 and Ralstonia eutropha were able to accumulate 26.76% and 28.97% of their dry cell weight when hydrolyzed grass was used as substrate and PHA accumulation increased to 41.7% and 42.2% when hydrolyzed seed was used as a sole carbon source.