Le Pham Tan Quoc, Le Thi Tra Giang, Nguyen Thi Ngoc Hanh, Giang Thuy Phuong Quyen and Nguyen Thi Phuong Thao
Biotechnology of Biotechnology
The aim of this study was the investigation on main factors (the grind time, ratio of water/peel and distillation time) affecting the amount of essential oil after distilling. Researching and optimizing the factors for essential oil production by laboratory – scale distillation and the yield was evaluated using the Response Surface Methodology (RSM) with Central Composite Face Centered (CCF) model.
There is the interaction between ratio of water/peel, distillation time and grind time with volume of essential oil extracted. Optimal result which is essential oil was 1.83 ml (y) with quantity of kumquat peel/batch of 50 g, grind time (x1) in 118 seconds, ratio of water/peel (x2) was 6/1, distillation time (x3) during 32 minutes at 100 oC. The essential oil from the peel of C.microcarpa (Hassk.) Bunge was analysed by GC-MS method. In which, there are a lot of compounds were useful, it can use widely in the food processing and the cosmetics industry, for instance limonene, α-Pinene, β-Pinene, β-Myrcene, Sabinen.
CCF, C. microcarpa (Hassk.) Bunge, essential oil, GC-MS, RSM