Abstract: Food and feed contamination by aflatoxins represents a great challenge for human and animal health. Aflatoxins detoxification using probiotic bacteria and yeasts has been introduced as an inexpensive and promising method. This article is organized with an overview of the potential application of probiotic bacteria and yeasts to eliminate, inactivate or reduce the bioavailability of aflatoxins, especially aflatoxin B1, in vitro and in vivo. Also, a fast glance to beneficial health effects and preservative properties of probiotics followed by the mechanism of binding of aflatoxins by probiotics, influence of different probiotic pretreatments, and the stability of aflatoxin-probiotic complexes are mentioned.