Abstract: In sheep’s and goat’s milk inoculated with a strain of Staphylococcus aureus, the staphylococcal growth was tested by the plate method according to EN ISO 6888-1 on Baird-Parker agar. The automated instrument miniVIDAS® was used for the detection of staphylococcal enterotoxin C by enzyme-linked immunofluorescent assay (ELFA). The influence of poor storage conditions (15 oC, 22 oC) and of the type of milk on the monitored parameters was evaluated. The results of the model experiments showed the dependence of the multiplication of S. aureus and subsequent production of staphylococcal enterotoxins on the culture/storage temperature and the type of milk. It is noteworthy that in raw milk, the S. aureus growth rate and production of enterotoxins can be suppressed by competitive microflora.