Marián Tomka*, Milan Chňapek, Jana Bradová, Zdenka Gálová
Biotechnology of Biotechnology
Identification and characterization of different genotypes is essential for improving the quality of cultivated varieties in breeding programs. Electrophoretic separation of barley storage proteins allows us to identify and to differentiate the individual genotypes. We used acid polyacrylamid gel electrophoresis (A-PAGE) and starch gel electrophoresis (SGE) to differentiate14 barley genotypes.Using SGE we were able to detect 29 lines while using APAGE we have detected only 22 lines. A total of 43 different alleles were deteceted and based on their combination we observed 20 hordein profiles using each method. The frequency of alleles varied from 0.51 % to 9.64 % for A-PAGE and from 1.0 % to 11.0 % for SGE. On the basis of detected alleles dendrograms were constructed using UPGMA algorithm. These dendrograms revealed genetic relations between varieties and divided collection of barley genotypes into two main groups based on A-PAGE and SGE. Both methods confirmed heterogeneity of barley varieties what allowed us to study intravarietal polymorphism.
barley, hordeins, polymorphism, genetic diversity, protein markers