Andrej Trebichalský, Alan Schulman, Ruslan Kalendar, Olga Stratula, Zdenka Gálová
Biotechnology of Biotechnology
Retrotransposons can be used for markers because their integration creates new joints between genomic DNA and their conserved ends. In our work, we had focused on finding of genetic relationships between winter triticale (X Triticosecale Witt.) and rye (Secale cereale L.) using retrotransposon-based markers. Together, five IRAP primers combinations and β-amylase (BAMY) genes were used for polymorphism detection of 37 cultivars of winter triticale and 5 cultivars of rye. From the obtained data, we had constructed UPGMA dendrogram, which differentiate samples on the basis of genetic relationships in two principal cluster and several subclusters. We had confirmed close relationships between cultivars Leontino and Pletomax, Trimmer and Wilfried or between cultivars Sorento and Amarillo 105.