February, 2014, vol. 3, special issue 2 (Biotechnology)
Želmíra Balážová, Andrej Trebichalský, Zdenka Gálová, Ruslan Kalendar, Alan Schulman, Olga Stratula, Milan Chňapek
Biotechnology of Biotechnology
The aim of our work was to detect genetic variability in the set of 59 winter and spring triticale (x Triticosecale Witt.) varieties using combination of 4 wheat SSR and 4 retrotransposon-based markers. The number of alleles for SSR markers ranged from 8 to 10 with an average number of 8,75 alleles per locus. For IRAP markers the number of alleles ranged from 9 to 10 with an average number of 9,25 alleles per locus Totally, 72 alleles were detected, 37 alleles for IRAP markers and 35 alleles for SSR markers. For the assessment of genetic diversity the dendrogram, based on the hierarchical cluster analysis using UPGMA algorithm was prepared. Fifty nine triticale cultivars were grouped into two major groups. The first group contained all winter triticale varieties and in the second cluster were included all spring triticale varieties. The closest relationship was found out between two Polish winter triticale cultivars, Alekto and Pizarro. Results showed the utility of combination of microsatellite and retrotransposon-based markers for estimation of genetic diversity of triticale genotypes leading to genotype identification.