February, 2014, vol. 3, special issue 2 (Biotechnology)
Yurij Slyvchuk, Ivan Hevkan, Iryna Matiukha, Vasyl Syrvatka
Biotechnology of Biotechnology
Complex studies of optimum quantitative and qualitative composition of carbohydrates and amino acids necessary for gonadotropines activity stabilization were the aim of our investigations. This was determined by biological active substances ─ amino acids: L- lysine , L- glycine and carbohydrates: sucrose and mannitol, which stabilize gonadotropins activity for longer use while preserving activity. As a result of the studies we found out that the addition 10 mg/sm3 of L-lysine and 75 mg/sm3 of sucrose for extended incubation provides the highest gonadotropin activity compared with other experimental and control batches of samples. In the experimental series of samples, where the stabilizer was added at a rate of 10 mg/sm3 L-lysine and 75 mg/sm3 mannitol, the activity was stable during 6 weeks of incubation and constituted more than 50% of the initial theoretical activity of hCG.