February 2015, vol. 4, special issue 2 (Biotechnology)
Dominika Ďurechová, Ildikó Matušíková, Jana Moravčíková, Martin Jopčík, Jana Libantová
Biotechnology of Biotechnology
Progress in biotechnology has generated an interest towards the isolation and characterization of genes that should be used in strengthening of plant defence against biotic and abiotic stresses. Here we focused on sequence analysis of the chitinase gene isolated from untraditional genetic source, carnivorous plant sundew (DroserarotundifoliaL.). The genomic sequence of DrChit indicated the coding region contained three exons and two introns.Splicing of introns predicted in silicowas confirmed by experimental approach. Moreover, RACE (rapid amplification cDNA ends) analysis revealed the transcription start sitelocated 41 bp upstream of the ATG start codon. This is one of the first reports onthe isolation and molecular characterization of chitinase gene from the carnivorous sundew plant.